Day 1 :
- Obesity: Current research | Obesity
Location: Conference Hall
Faculty of Medicine, Lomonosov Moscow State University, Russia
Maria Evseeva is a PhD student in the Moscow State University. The focus of her research is the transcriptional regulation in adipogenesis and obesity. Specific interests include metabolic syndrome, non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes.
Statement of the Problem:
Obesity is a major health problem and is associated with a high risk of metabolic diseases, for instance type 2 diabetes. Since pathological adipogenesis causes obesity-related complications, an understanding of the molecular mechanisms, involved in physiological and pathological adipogenesis can help to develop new strategies to prevent or cure obesity and related diseases.
Previously, we have shown that transcription factor Hhex (PRH) knockdown blocks adipogenesis in 3T3L1 preadipocytes in a dose-dependent manner and leads to a significant decrease of PPAR-gamma protein - the main regulator of adipogenesis - while the PPARG mRNA level remains unaffected. We have also shown that these effects are proteasome independent.
Here we study the role of Hhex in human MSCs adipogenesis and show that in human MSCs Hhex knockdown causes similar effects: suppresses adipogenesis dose-dependently and downregulates PPAR gamma protein.
The obtained results support the idea that Hhex is important regulator of adipogenesis and may be a perspective target for future drug discovery.
Methodology & Theoretical Orientation: human MSCs were obtained from healthy donors and cultured in DMEM medium, supplied with 10% fetal bovine serum and 1% antibiotics at 37C and 5% CO2. The siRNA to Hhex and control siRNA were transduced to cells via lentiviral particles. GFP-positive cells were selected by cell-sorting and induced to the adipogenic differentiation. After 21 days cells were harvested and the efficacy of adipogenic differentiation was analyzed with Oil Red O staining, PCR-RT and western-blotting analysis.
Findings: Here we show that in human MSCs Hhex knockdown suppresses adipogenesis dose-dependently and downregulates PPAR gamma protein with no effect on mRNA level. These results reproduce the effects which we have reported previously in 3T3L1 preadipocytes.
Conclusion & Significance: The obtained results support the idea that Hhex is an important regulator of adipogenesis and may be a perspective target for future drug discovery.
Assistant Professor of Physical Medicine & Rehabilitation, Albert Einstein College of Medicine, NY Professor of Physical Medicine, Rheumatology & Rehabilitation, Alexandria University, Egypt
Obesity is one of the most common and important risk factors for cardiovascular diseases. That is equally applicable for both sexes. Obesity, itself is considered independent risk factor as well as lead to exaggeration and amplifications of other common risk factors as diabetes, dyslipidemia and hypertension. Evaluation of obesity, and different interventional methods should be part of any structured cardiac rehabilitation programs.
Obesity has been determined to be an important risk factor for cardiovascular disease among men and women, although specific data on racial/ethnic minorities are lacking.
Obesity appears to interact with or amplify the effects of other risk factors by mechanisms that as yet remain frontier for further research.
Alarming data from the National health and Nutrition exam surveys show that the prevalence of obesity among Americans has increased over the past 20 years, such that an estimated 47 million adults American are deemed to be overweight.
Thus, obesity should be viewed vas a prevalent, serious, and, to date, refractory health problem.
In large consecutive series of patients enrolled in cardiac rehabilitation programs, the prevalence of obesity in nearly 50%.
Surprisingly, weight management and obesity, despite their strong link to other risk factors, have not been a primary focus in cardiac rehabilitation. This is evidenced by the sparsity of scientific data of this subject in cardiac rehabilitation literature.
It is very clear however, that more data are needed and that additional focused interventions must be performed