Alphonse Laya
University of Maroua, Cameroon
Title: Phenolics (bound and free) in edible cassava (Manihot esculenta Crantz) leaves harvested at different time affected by in vitro gastrointestinal digestion and their antidiabetic (α-amylase and α-glucosidase) and anti-obesity (lipase) inhibitory activities
Biography
Biography: Alphonse Laya
Abstract
Edible cassava leaves (Manihot esculenta) were consumed as vegetable for their bioactive compounds, however the effect of harvesting time on these bioactive compounds and their antidiabetic and anti-obesity inhibitory activities were scarcely reported in the literature. Phenolics from edible leaves harvested at different time were assessed using HPLC-DAD, and their antidiabetic and anti-obesity inhibitory activities were investigated before and after in vitro gastrointestinal digestion. The results showed significant (P<0.05) effect of harvesting time on phenolic contents. Bound phenolics showed higher lipase inhibitory activity than their free fractions. However, phenolic contents decreased after in vitro digestion, while they showed high antidiabetic and anti-obesity inhibitory activities. The present study demonstrated that edible leaves harvested at different times are rich in flavonoids, stilbenes, tannins and saponins. These bioactive compounds are highly potent against α-amylase, α-glucosidase and lipase before and after in vitro gastrointestinal digestion. Therefore, we recommend edible cassava leaves for important biological active compounds for the prevention of obesity and type 2 diabetes.
Keywords: Bound phenolic, in vitro gastrointestinal digestion, antidiabetic, Manihot esculenta, harvest time, type 2 diabetes.